BioinformaticsBMC GenomicsPeer-reviewed
Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing
Benjamin P. Kellman, Hratch Baghdassarian, Tiziano Pramparo…·Nathan E. Lewis
This study quantifies how repeated freeze-thaw cycles affect RNA-Seq reliability, showing that poly(A)-enriched samples lose reproducibility after three cycles.
32 citations