BioinformaticsbioRxivPreprint
KuafuPrimer: Machine learning empowers the design of 16S amplicon sequencing primers toward minimal bias for bacterial communities
Haoyu Zhang, Xiaoqing Jiang, Xiongwu Yu…·Huaiqiu Zhu
KuafuPrimer uses machine learning to design 16S rRNA gene primers with minimal bias, improving taxonomic accuracy and detecting rare taxa missed by universal primers.
DOI 10.64898/2026.03.29.714677