Performance characteristics of next-generation sequencing for antimicrobial resistance gene detection in genomes and metagenomes
Ashley M. Rooney, Amogelang R. Raphenya, Roberto Gustavo Melano, Christine Seah, N. Yee, Derek R. MacFadden, Andrew G. McArthur, Pierre H. H. Schneeberger, Bryan A. Coburn
This study evaluates the performance of short-read sequencing for detecting antimicrobial resistance genes in genomes and metagenomes, finding that assembly-based methods require 15X coverage for reliable detection.
bioRxiv · Jun 25, 2021 · PreprintBioinformatics