Releasing Protein Cargo from Synthetic Cells by Coupling Cell-Free Expression to Osmotic Actuation
Hongfei Liu, Jérémie J. M. Marlhens, Simon Schubotz, Heinz Koeppl
This study demonstrates that osmotic stress from internal gene expression can promote macromolecular release from synthetic cells without requiring membrane pores.
bioRxiv · Oct 7, 2026 · PreprintSynthetic Biology