Unlocking karst biodiversity with eDNA: a validated qPCR toolkit for discovery and monitoring the worlds largest cavefish radiation (Cyprinidae: Sinocyclocheilus)
Yunxiang Peng, Shenjie Yu, Rongjie Chen, Yewei Liu, Tingru Mao, Ting Lin, Dan Sun, Marcio Roberto Pie, Jian Yang, Madhava Meegaskumbura
The paper develops and validates a qPCR toolkit for detecting and identifying Sinocyclocheilus cavefish using eDNA, showing high specificity and detection limits in both lab and field settings.
bioRxiv · Dec 11, 2025 · PreprintEvolutionary Biology