MicrobiologyNucleic Acids ResearchPeer-reviewed
Visualization of Type IV-A1 CRISPR-mediated repression of gene expression and plasmid replication
Mariana Sanchez-Londono, Selina Rust, Rogelio Hernández-Tamayo…·Lennart Randau
This study examines the CRISPRi effects of the Type IV-A1 CRISPR-Cas system in Pseudomonas oleovorans and Escherichia coli using synthetic crRNAs, revealing long-range gene repression and plasmid targeting.