Cancer BiologyBiology OpenPeer-reviewed
CRISPR-Cas9 RALA knockout and reconstitution. Detection and role of RALA S194 phosphorylation in RAS-dependent and RAS-independent cancers.
Mayuresh Vishwas Konde, Siddhi Inchanalkar, Tushar Sherkhane…·Nagaraj Balasubramanian
The paper investigates the role of RALA S194 phosphorylation in cancer by knocking out RALA and reconstituting it with wild-type and mutant forms, finding that phosphorylation is necessary for tumor formation but not activation.
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