Resolving eukaryotic river biofilm communities using long-read sequencing for biomonitoring
Meri A. J. Anderson, Amy C. Thorpe, Susheel Bhanu Busi…·Daniel S. Read
This study compares short-read and long-read sequencing of 18S rRNA gene amplicons in river biofilms, showing that long-read sequencing improves taxonomic resolution while trimming can introduce mismatches.