Design, Fabrication, and Theoretical Investigation of a Cost-Effective Laser Printing Based Colorimetric Paper Sensor for Non-Invasive Glucose and Ketone Detection

Abstract

Diabetes, a chronic condition, is one of the prevalent afflictions of the 21st century, and if left unchecked, this ailment could lead to severe life-threatening complications. A widely accepted methodology for monitoring diabetes is the estimation of the glucose and ketone contents in the body-fluids, viz. blood, urine, etc. Additionally, certain conditions such as starvation, and following a protein rich diet (e.g., keto-diet) could also lead to significant changes in the ketone content, thereby resulting in false-positive diagnosis. Hence, a precise, portable, and on-demand procedure for the rapid and combined estimation of glucose and ketone in the bodily-fluids is of utmost importance. To that end, paper-based analytical devices (PADs) are promising tools, owing to their multitudinous advantages, and compatibility with biofluids. Although, numerous researchers have contributed substantially in the fundamental investigation, design, and fabrication of PADs for various applications, a combined platform capable of rapid, accurate and on-demand glucose and ketone detection, that is easy to fabricate, is still relatively unexplored. Moreover, the flow dynamics of an analyte, in combination with enzyme-catalysed (for glucose) and uncatalyzed reactions (for ketone), within a porous paper matrix is also vaguely understood. Herein, we present a facile laser-printing based fabrication of colorimetric sensors on a filter paper, for rapid, and non-invasive estimation of glucose and ketone contents in urine. The urine sample, upon being deposited in a particular expanse, is wicked through the paper matrix, and reacts with specific reagents in the designated zone(s), giving rise to a final color, concomitant with the glucose or ketone content in the sample. The device design enables the liquid to be wicked into the porous matrix in a way that would concentrate the colored product in a dedicated detection zone, thereby augmenting the feasibility for accurate colorimetric detection. Furthermore, we present for the first time, a detailed dynamic model of the flow-field in a variable cross-section paper device using the Richards equation, while also considering the species transport and reaction kinetics within the porous media. The results of the numerical simulation agree well with those observed experimentally, thereby validating the present model. Finally, we also developed a web and desktop-based application that would enable the user to upload the images of the colored zones to provide an accurate estimate of the glucose and ketone content in the sample. We believe that our model, in combination with the proposed fabrication methodology, and the in-house developed app., would enable rapid and reliable fabrication of PADs for various fundamental investigations, and applications pertaining to affordable health-care monitoring. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=59 SRC="FIGDIR/small/461386v1_ufig1.gif" ALT="Figure 1"> View larger version (28K): org.highwire.dtl.DTLVardef@49e824org.highwire.dtl.DTLVardef@27a512org.highwire.dtl.DTLVardef@c65242org.highwire.dtl.DTLVardef@429896_HPS_FORMAT_FIGEXP M_FIG C_FIG