bioRxivEcology
Expedited Assessment of Terrestrial Arthropod Diversity by Coupling Malaise Traps with DNA Barcoding
J. R. deWaard, V. Levesque-Beaudin, S. L. deWaard, N. V. Ivanova, J. T. A. McKeown, R. Miskie, S. Naik, K. Perez, S. Ratnasingham, C. N. Sobel, J. E. Sones, C. Steinke, A. C. Telfer, A. D. Young, M. R. Young, E. V. Zakharov, P. D. N. Hebert
Abstract
O_LIMonitoring changes in terrestrial arthropod communities over space and time requires a dramatic increase in the speed and accuracy of processing samples that cannot be achieved with morphological approaches.\nC_LIO_LIThe combination of DNA barcoding and Malaise traps allows expedited, comprehensive inventories of species abundance whose cost will rapidly decline as high-throughput sequencing technologies advance.\nC_LIO_LIAside from detailing protocols from specimen sorting to data release, this paper describes their use in a survey of arthropod diversity in a national park that examined 20,000 specimens representing 2200 species.\nC_LIO_LIThese protocols can support arthropod monitoring programs at regional, national, and continental scales.\nC_LI
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