Multimodal social context modulates behavior in larval Drosophila

Abstract

All animals need to navigate and make decisions in social environments. They influence each others behavior, but how important this is and how they process and represent social information in their brain is less well understood. This includes fruit flies and fly larvae, which are usually not known as "social insects". Using a Drosophila larva assay with reduced stimulation, we found that larval groups show enhanced dispersal and distance from each other in the absence of food. This social context-dependent modulation overrides responses to other external sensory cues and is shaped by developmental social experience. Leveraging the genetic toolbox available in Drosophila, we find that different sensory modalities are required for normal social context modulation. Our results show that even less social animals like fly larvae are affected by conspecifics and that they recognize each other through multimodal sensory cues. This study provides a tractable system for future dissection of the neural circuit mechanisms underlying social interactions.