Siphonous green macroalgae with contrasting capacities for the energy-dependent quenching, qE, rely on different photoprotective mechanisms

Abstract

To combat light-induced damage, photosynthetic organisms have evolved several photoprotective mechanisms, including non-photochemical quenching (NPQ) of excess light energy. Bryopsidales green macroalgae lack the energy-dependent quenching (qE) and can therefore induce NPQ only slowly. We illuminated a Bryopsidales macroalga (Bryopsis sp.) and a morphologically similar Dasycladales alga (Acetabularia acetabulum), capable of qE, with high light. No differences in the rate of Photosystem II (PSII) damage, probed by the chlorophyll a fluorescence parameter FV/FM in the absence and presence of lincomycin, were observed between constant and fluctuating light in Bryopsis sp., nor between the two algae. In Bryopsis sp., however, photoinhibition lead to decreased rates of electron transfer (estimated by fluorescence and oxygen evolution), while a stimulation was observed in A. acetabulum. Compared to A. acetabulum, Bryopsis sp. showed slow PSII repair, but as the rate of repair increased with increasing rates of the damage, the slow repair may not indicate increased oxidative stress but be a regulatory response. We observed, in A. acetabulum, a post-illumination dip in PSII activity and a concurring bump in NPQ, which were removed by an inhibitor of the NDA2-dependent cyclic electron transfer route and enhanced in the presence of inhibitor of mitochondrial respiration, suggesting that these pathways reduce and oxidise, respectively, the plastoquinone pool in the dark in this alga. Nigericin, which prevents the formation of proton gradient and thus qE, increased photoinhibition in A. acetabulum but not in Bryopsis sp.. Anoxia and inhibitors of the plastid terminal oxidase and mitochondrial respiration, on the other hand, enhanced photoinhibition only in Bryopsis sp., suggesting that oxygen-dependent pathways (including flavodiiron proteins) are important for photoprotection in the qE-deficient Bryopsidales algae.