Hyperglycemia and Cancer. Human lung carcinoma by means of Raman spectroscopy and imaging

Abstract

Raman spectroscopy and Raman imaging allow to identify the biochemical and structural features of human cancer lung cell line (CCL-185) and the cell line supplemented with glucose and deuterated glucose in normal and hyperglycemia conditions. We found that isotope substitution of glucose by deuterated glucose allows to separate de novo lipid synthesis from exogenous uptake of lipids obtained from the diet. We demonstrated that glucose is largely utilized for de novo lipid synthesis. Our results provide a direct evidence that high level of glucose decreases the metabolism via oxidative phosporylation in mitochondria in cancer cells and shifts the metabolism to glycolysis via Wartburg effect. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=163 SRC="FIGDIR/small/487128v1_ufig1.gif" ALT="Figure 1"> View larger version (51K): org.highwire.dtl.DTLVardef@1363c82org.highwire.dtl.DTLVardef@1beb1cborg.highwire.dtl.DTLVardef@8aa1fdorg.highwire.dtl.DTLVardef@19127e5_HPS_FORMAT_FIGEXP M_FIG C_FIG