First detection of Crimean Congo Hemorrhagic Fever antibodies in cattle and wild fauna of southern continental France: investigation of explicative factors

Abstract

Crimean-Congo Haemorrhagic Fever (CCHF) is a tick-borne zoonosis with significant public health implications due to its expanding geographic distribution and potential for severe outcomes in humans. This study represents the first serological survey conducted in mainland France to detect antibodies against the Crimean-Congo Haemorrhagic Fever Virus (CCHFV) in domestic and wild fauna, providing critical insights into the viruss circulation. We analyzed 8,609 cattle sera and 2,182 wildlife sera collected across the French Mediterranean region from 2008 to 2022, using enzyme-linked immunosorbent assays (ELISA) and pseudo-plaque reduction neutralization tests (PPRNT) for antibody detection and confirmation. Seropositivity was detected in both cattle (2.04%) and wildlife (2.25%), with higher rates observed in specific regions such as Pyrenees-Orientales and Hautes-Pyrenees. These findings reveal spatial clusters of CCHFV circulation and suggest the existence of enzootic transmission cycles involving local tick vectors and animal hosts. Our multivariate analysis identified key factors influencing seropositivity, including animal age, habitat characteristics, and potential wildlife interactions. The presence of natural open habitats and coniferous forests was significantly associated with higher seropositivity in cattle, while sex and geographical variability played a role in wildlife seroprevalence. These findings highlight the importance of environmental and anthropogenic factors in shaping the dynamics of CCHFV transmission. This study demonstrates that CCHFV is actively circulating in parts of mainland France, emphasizing the need for enhanced surveillance and integrated approaches to monitor zoonotic pathogens. It also raises questions about the role of additional tick vectors, such as Hyalomma lusitanicum, in the transmission cycle. These results contribute to a better understanding of CCHF epidemiology and offer valuable guidance for public health strategies to mitigate the risks associated with this emerging disease. Author SummaryCrimean-Congo Haemorrhagic Fever (CCHF) is a severe disease spread by ticks that affects humans and animals. Although the disease is largely distributed in parts of Europe, Africa, and Asia, its presence in France has been uncertain. My study investigated whether the virus causing this disease is circulating in southern mainland France by testing blood samples from domestic animals, like cattle, and wild animals, such as deer and wild boars, for signs of previous infection. I found evidence of the virus in several regions, particularly in the Pyrenees-Orientales, Hautes-Pyrenees and Alpes-maritimes, suggesting that the virus is indeed circulating among animals and ticks in some parts of mainland France. By studying where infected animals were found and considering factors such as age, habitat, and environmental conditions, I identified that older animals seem to have been more often exposed to the virus, as well as animals frequenting open environments favorable to ticks. These findings are important because they show that the disease could potentially spread to new areas and affect human populations. My work highlights the need for ongoing monitoring of ticks and animals for CCHFV epidemiological surveillance and the protection of public health.