Salicylic acid-related ribosomal protein CaSLP improves drought and Pst. DC3000 tolerance in pepper

Abstract

The ribosomal protein SA plays an essential role in multiple aspects and is involved in plant growth and response to various stresses. Drought threatens pepper yield and quality. However, the resistance mechanism of pepper in response to drought are complex and not yet fully understood. Here, we describe the role of CaSLP in mediating pepper tolerance to drought stress. we found that CaSLP was highly expressed under drought and salicylic acid (SA) stress, and CaSLP was localized in cell nucleus and cytomembrane. Knockout of CaSLP gene significantly decreased the pepper drought tolerance, while transient expression of CaSLP leads to drought tolerance in pepper, and overexpression of the CaSLP dramatically increased the drought stress tolerance in Arabidopsis. Furthermore, exogenous spring salicylic acid enhanced drought tolerance. The characterization of resistance molecular mechanisms in the Pseudomonas syringae pv. Tomato DC3000 (Pst.DC3000) is of great significance for the pepper yield and quality, we found that CaSLP-knockdown pepper plants demonstrated decreased Pst.DC3000 tolerance, whereas ectopic expression of the CaSLP increased the Pst.DC3000 stress tolerance in Arabidopsis. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) results showed that CaNAC035 physically interacts with CaSLP in the cell nucleus, and the CaNAC035 was identified as an upstream partner of the CaPR1 promoter and activated the transcription. Taken together, our data demonstrated that CaSLP plays an essential role in the regulation of drought stress. Our study elucidates the roles of CaSLP response to drought stress tolerance. Furthermore, a possible regulatory model and molecular mechanisms under drought stress is proposed.