Acute Sprint Exercise Transcriptome in Human Adipose Tissue: Associations with Growth Hormone

Abstract

It was hypothesised that sprint exercise induces changes in adipose tissue (AT) gene expression related to lipolysis, and that growth hormone (GH) acts as a stimulus. Twelve healthy males and females perform 3x30-s all-out cycle sprints (SIT) and six unloaded cycling (CON). AT biopsies were performed pre and 2 hours post-exercise. Serum GH response was greater in SIT than CON, but no enrichment of differentially expressed genes (DEGs) related to lipolysis was found in AT. However, the GH-receptor was one of few DEGs in AT with interaction between SIT and CON and exercise-induced changes in expression of the pre-selected GH-related targets, CISH, PTEN and G0S2 were associated with exercise-induced increase in GH. This supports a GH-mediated effect of SIT on lipolysis. In a genome-wide analysis, exercise-induced changes in expression of ARHGAP24, TAF4B, ARID5B, MIR604 and MIR938, were associated with increase in GH. The function of these genes is not well understood, but some relationship to AT has earlier been demonstrated Sex influenced GH-response to exercise and expression of the GH-responsive gene CISH. Among the most downregulated genes were the core clock genes PER1, NR1D1 and CIART, which decreased in both SIT and CON. This underscores the necessity to control for diurnal variations and sex in future exercise studies on regulation of AT lipolysis.