bioRxivBioengineeringPreprint
Back to the Future: Unleashing your cytometers spectral potential
R. V. Walker, C. Hall, H. Ibrahim, S. Thompson, P. Hobson, J.-A. Crofts, P. Nobes, S. Lim, T. Burpee
Abstract
With the recent growth in spectral flow cytometry many laboratories are investing in new spectral flow cytometers in order to maximise the information gathered about every cell. This study hypothesised that traditional cytometers already within many laboratories may be used as spectral cytometers and have shown using a range of different cytometers that data acquired may be unmixed after acquisition.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level
Oliver T. Burton, Leoni Bücken, … Adrian Liston·bioRxiv·Oct 27, 2025
- The consequences of mismatched buffers in spectral cell sorting
Rita A. S. Dapaah, Laura Ferrer‐Font, … Rachael V. Walker·Cytometry Part APeer-reviewed·Nov 30, 2024
- Protocol to optimize gain settings of Aurora for better data resolution
Debajit Bhowmick, Lindsay Richard, Michelle Leigh Ratliff·bioRxiv·Jun 11, 2025
- Measurement and prediction of unmixing-dependent spreading in spectral flow cytometry panels
Peter L. Mage, A Konecny, Florian Mair·bioRxiv·Apr 17, 2025
- Panel Perils: How Size, Fluorochrome Choices, and Unmixing Algorithms Shape Your Analysis Adventure!
Debajit Bhowmick, Timothy P. Bushnell·bioRxiv·Jun 11, 2025
- Design and Optimization Protocol for High-Dimensional Immunophenotyping Assays using Spectral Flow Cytometry
L. Ferrer-Font, C. Pellefigues, … K. M. Price·bioRxiv·Sep 27, 2019