S. Thompson

FACS-Sortable Triple Emulsion Picoreactors for Screening Reactions in Biphasic Environments

This paper develops FACS-sortable triple emulsion picoreactors for high-throughput screening of biphasic reactions, demonstrating stability and compatibility with protein synthesis.

Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins

The paper introduces TXTL-QCMD, a method to dynamically characterize membrane-interacting proteins without purification or labeling, revealing insights into IIMP-membrane interactions.

The Genetic Landscape of a Metabolic Interaction

The paper quantifies how DHFR mutations affect TYMS growth rates using deep mutational scanning, revealing metabolic context's role in enzyme mutational tolerance.

Back to the Future: Unleashing your cytometers spectral potential

This paper demonstrates that traditional flow cytometers can be used as spectral cytometers by unmixing data acquired from them.

Genetic architecture and evolution of color variation in American black bears

This study identifies a missense mutation in TYRP1 responsible for the cinnamon color morph in American black bears and traces its evolutionary origin and spread.

Linking collective migration/growth to differentiation boosts global shaping of the transcriptome and exhibits a grasshopper effect for driving maturation

The paper links collective migration to differentiation in hPSC-derived hepatocytes, showing a significant increase in maturation through a deep-learning gene circuit.

Mucin-degrading gut bacteria promote anti-parasitic immunity

This study shows that mucin-degrading gut bacteria influence anti-parasitic immunity by modulating host immune responses, with dietary fiber affecting this interaction.

Antibacterial potency of Type VI amidase effector toxins is dependent on substrate topology and cellular context

This study identifies functional determinants of Tae1, a bacterial T6SS amidase toxin, showing that its antimicrobial potency depends on substrate topology and cellular context.

Single-molecule nanopore dielectrophoretic trapping of α-Synuclein with lipid membranes

This paper uses single-molecule nanopore dielectrophoresis to study α-Synuclein folding and unfolding in lipid membranes and how metal ions and peptidomimetics affect these processes.

Facultative variation across a shallow to deep torpor spectrum in hummingbirds

This study shows hummingbirds use both deep and shallow torpor, indicating a flexible physiological response to energy and environmental needs.

Genomic epidemiology of Mycobacterium bovis infection in sympatric badger and cattle populations in Northern Ireland.

This study sequenced M. bovis isolates from badgers and cattle in Northern Ireland to analyze genomic epidemiology, finding an endemic lineage with low diversity and evidence of interspecies transmission.

Structurally Distributed Surface Sites Tune Allosteric Regulation

The paper investigates how mutations affect allosteric regulation by analyzing the impact of 1548 DHFR single mutations on allostery using a high-throughput assay.

Effects of physiological deficits in pineal melatonin on Triple Negative Breast Cancer.

This study investigates how melatonin deficits affect Triple Negative Breast Cancer (TNBC) risk and progression using in vitro and in vivo models.

FOXA1/2 depletion drives global reprogramming of differentiation state and metabolism in a human liver cell line and inhibits differentiation of human stem cell-derived hepatic progenitor cells

This study shows that depletion of FOXA1/2 in human liver cells reprograms differentiation state and metabolism, promoting de-differentiation and pluripotency.

Modulating the cellular context broadly reshapes the mutational landscape of a model enzyme

The paper shows that the absence of the protease Lon in E. coli reshapes the mutational landscape of DHFR, increasing the proportion of advantageous mutations.

Flex ddG: Rosetta ensemble-based estimation of changes in protein-protein binding affinity upon mutation

The paper introduces flex ddG, a method using Rosetta to estimate binding affinity changes upon mutation by sampling conformational diversity, and shows it outperforms existing methods in several mutation types.