A practical framework RNMF for the potential mechanism of cancer progression with the analysis of genes cumulative contribution abundance

Mutational signatures can reveal the mechanism of tumorigenesis. We developed the RNMF software for mutational signatures analysis, including a key model of cumulative contribution abundance (CCA) which was designed to highlight the association between genes and mutational signatures. Applied it to 1073 esophageal squamous cell carcinoma (ESCC) and found that APOBEC signatures (SBS2* and SBS13*) mediated the occurrence of PIK3CA E545k mutation. Furthermore, we found that age signature is strongly linked to the TP53 R342* mutation. In addition, the CCA matrix image data of genes in the signatures New, SBS3* and SBS17b* were helpful for the preliminary evaluation of shortened survival outcome. In a word, RNMF can successfully achieve the correlation analysis of genes and mutational signatures, proving a strong theoretical basis for the study of tumor occurrence and development mechanism and clinical adjuvant medicine.