Cell ReportsGeneticsPeer-reviewed journal article
CRISPR-Cas9 Gene Editing in Lizards Through Microinjection of Unfertilized Oocytes
Ashley M. RasysiD, Sungdae Park, Rebecca BalliD, Aaron J. AlcalaiD, James D. LauderdaleiD, Douglas B. MenkeiD
Abstract
CRISPR-Cas9 mediated gene editing has enabled the direct manipulation of gene function in many species. However, the reproductive biology of reptiles presents unique barriers for the use of this technology, and there are currently no reptiles with effective methods for targeted mutagenesis. Here we present a new approach that enables the efficient production of CRISPR-Cas9 induced mutations in Anolis lizards, an important model for studies of reptile evolution and development.
- Citations
- 116
- FWCI
- 4.55
- Field percentile
- top 4%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Genetic modification of the ant Lasius niger using CRISPR-Cas9 technology
Mauno Konu, Jonna Kulmuni, Lumi Viljakainen·Insect Molecular BiologyPeer-reviewed·Aug 28, 2022
- CRISPR/Cas9-mediated generation of biallelic G0 anemonefish (Amphiprion ocellaris) mutants
Laurie J. Mitchell, Valerio Tettamanti, … Fabio Cortesi·bioRxiv·Oct 7, 2020
- Germline mutagenesis of Nasonia vitripennis through ovarian delivery of CRISPR-Cas9 ribonucleoprotein
Duverney Chaverra‐Rodriguez, Elena Dalla Benetta, … Omar S. Akbari·Insect Molecular BiologyPeer-reviewed·Jul 27, 2020
- Generation of heritable germline mutations in the jewel wasp Nasonia vitri pennis using CRISPR/Cas9
Ming Li, Lauren Au, … Omar S. Akbari·Scientific ReportsPeer-reviewed·Apr 10, 2017
- Gene editing without a genome: generation and validation of F0 CRISPR mutants in gastropod mollusc Crepidula fornicata
Danielle C. Jordan, Molly L. Rivers, … Victoria A. Sleight·Developmental Biology AdvancesPeer-reviewed·May 14, 2026
- Development of Highly Efficient CRISPR-Mediated Gene Editing in the Rotifer Brachionus manjavacas
Haiyang Feng, Gemma Bavister, … David B. Mark Welch·bioRxiv·Oct 25, 2022