The sound of restored soil: Measuring soil biodiversity in a forest restoration chronosequence with ecoacoustics

Abstract

Forest restoration requires monitoring to assess changes in above- and below-ground communities, which is challenging due to practical and resource limitations. With emerging sound recording technologies, ecological acoustic survey methods--also known as ecoacoustics--are increasingly available. These provide a rapid, effective, and non-intrusive means of monitoring biodiversity. Above-ground ecoacoustics is increasingly widespread, but soil ecoacoustics has yet to be utilised in restoration despite its demonstrable effectiveness at detecting meso- and macrofauna acoustic signals. This study applied ecoacoustic tools and indices (Acoustic Complexity Index, Normalised Difference Soundscape Index, and Bioacoustic Index) to measure above- and below-ground biodiversity in a forest restoration chronosequence. We hypothesised that higher acoustic complexity, diversity and high-frequency to low-frequency ratio would be detected in restored forest plots. We collected n = 198 below-ground samples and n = 180 ambient and controlled samples from three recently degraded (within 10 years) and three restored (30-51 years ago) deciduous forest plots across three monthly visits. We used passive acoustic monitoring to record above-ground biological sounds and a below-ground sampling device and sound-attenuation chamber to record soil communities. We found that restored plot acoustic complexity and diversity were higher in the sound-attenuation chamber soil but not in situ or above-ground samples. Moreover, we found that restored plots had a significantly greater high-frequency to low-frequency ratio for soil, but no such association for above-ground samples. Our results suggest that ecoacoustics has the potential to monitor below-ground biodiversity, adding to the restoration ecologists toolkit and supporting global ecosystem recovery. Implications for PracticeO_LIThis is the first known study to assess the sounds of soil biodiversity in a forest restoration context, paving the way for more comprehensive studies and practical applications to support global ecosystem recovery. C_LIO_LISoil ecoacoustics has the potential to support restoration ecology/biodiversity assessments, providing a minimally intrusive, cost-effective and rapid surveying tool. The methods are also relatively simple to learn and apply. C_LIO_LIEcoacoustics can contribute toward overcoming the profound challenge of quantifying the effectiveness (i.e., success) of forest restoration interventions in reinstating target species, functions and so-called services and reducing disturbance. C_LI