Green Fabricated Zinc Oxide Nanoformulated Media Enhanced Callus Induction and Regeneration Dynamics of Panicum virgatum L.

Abstract

The current study was focused on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. Here, we synthesized zinc oxide nanoparticles through Cymbopogon citratus leaves extract to evaluate the influence of zinc oxide nanoparticles on the quality of plant regeneration in switchgrass. X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) validate phase purity of green synthesize Zinc oxide nanoparticles whereas, electron microscopy (SEM) has illustrated the average size of particle 50{+/-}4 nm with hexagonal rod like shape. Energy dispersive Xray (EDS) spectra depict major peaks of Zn (92.68%) while minor peaks refer to Oxygen (7.32%). ZnO NPs demonstrate the incredibly promising results against callogenesis. Biosynthesized ZnO NPs at optimum concentration showed very promising effect on plant regeneration ability. Both the explants, seeds and nodes used in study showed dose dependent response and upon high doses exceeding 40 mg/L the results were recorded negative, whereas at 30 mg/L both explants demonstrate 70 % and 76 % regeneration frequency. The results conclude that zinc oxide nanoparticles enhance plant growth and development. Being one of the essential plant nutrients, ZnO has greatly tailored the nutritive properties at nano-scale.