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bioRxivPlant BiologyPreprint

In vitro floral development in poplar: Insights into seed trichome and trimonoecy regulation

M. A. Ortega, R. Zhou, M. S. S. Chen, W. P. Bewg, B. Simon, C.-J. Tsai

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Abstract

Woody perennials including Populus spp. (poplars) have a juvenile phase that ranges from several years to decades in length. This and the year-long floral development process are major impediments to breeding and to fundamental research of reproductive traits. Here we report a CRISPR-empowered in vitro flowering system and demonstrate its application using three reproductive traits: sex, seed trichomes, and a previously undescribed potential for trimonoecy in poplar.

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