C.-J. Tsai

Active state structures of a bistable visual opsin bound to G proteins

This study presents active state structures of a bistable opsin, JSR1, bound to G proteins, revealing differences in activation mechanisms between monostable and bistable opsins.

Human Cells for Human Proteins: Isotope Labeling in Mammalian Cells for Functional NMR Studies of Disease-Relevant Proteins

The paper develops cost-effective isotope labeling protocols in HEK293 cells for NMR studies of difficult-to-produce human proteins, enabling detailed structural and functional analysis.

Activating an invertebrate bistable opsin with the all-trans 6.11 retinal analogue

This study shows that the all-trans 6.11 retinal analogue activates the invertebrate bistable opsin JSR1 and enables complex formation with downstream signaling partners.

Perturbation of tonoplast sucrose transport alters carbohydrate utilization for seasonalgrowth and defense metabolism in coppiced poplar

This study shows that SUT4 modulates seasonal trade-offs between growth and defense metabolism in poplar by affecting carbohydrate utilization during coppicing.

In vitro floral development in poplar: Insights into seed trichome and trimonoecy regulation

The paper develops an in vitro flowering system in poplar to study reproductive traits like sex, seed trichomes, and trimonoecy.

ULTRAFAST STRUCTURAL CHANGES DIRECT THE FIRST MOLECULAR EVENTS OF VISION

This study uses ultrafast crystallography to reveal how photoactivated retinal initiates structural changes in rhodopsin, leading to GPCR activation and vision.

Multiplex editing of the Nucleoredoxin1 tandem array in poplar: from small indels to translocations and complex inversions

This study uses CRISPR-Cas9 to edit a tandem array of seven NRX1 genes in poplar, generating diverse mutations including indels, deletions, translocations, and inversions.

Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA

A single gRNA targeting conserved sequences enabled efficient mutagenesis of a multigene family, resulting in trichomeless and triterpene-free poplar mutants.

Synthetic dysmobility screen unveils an integrated STK40-YAP-MAPK system driving cell migration

The paper identifies an integrated STK40-YAP-MAPK system that regulates cell migration and introduces synthetic dysmobility as a strategy to disrupt migration by blocking collaborative pathways.

Structural basis of the activation of the CC chemokine receptor 5 by a chemokine agonist

The paper reports the cryo-EM structure of CCR5 bound to a chemokine agonist and Gi protein, revealing its activation mechanism.

Plasma Membrane Phylloquinone Biosynthesis in Nonphotosynthetic Parasitic Plants

The paper identifies plasma membrane phylloquinone biosynthesis in nonphotosynthetic parasitic plants and suggests its role in transmembrane redox signaling.

Defoliation-induced compensatory transpiration is compromised in SUT4-RNAi Populus

This study examines how impaired sucrose trafficking in poplars affects compensatory transpiration after defoliation, finding that PtaSUT4 facilitates water uptake adjustments.

Compensatory guaiacyl lignin biosynthesis at the expense of syringyl lignin in 4CL1-knockout poplar

Knockout of 4CL1 in Populus reveals a 4CL5-dependent compensatory pathway for lignification with links to thiol redox balance and sulfur assimilation.

Cryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the Gβ subunit

The paper presents a cryo-EM structure of the rhodopsin-Gαi-βγ complex, revealing the C-terminal tail of rhodopsin bound to the Gβ subunit and identifying common anchoring points across G protein-subtype interactions.

Shared expression of Crassulacean acid metabolism (CAM) genes predates the origin of CAM in the genus Yucca.

The paper identifies shared expression patterns of CAM genes in C3 and CAM Yucca species, suggesting ancestral propensity for CAM.