Soil-microbes-mediated invasional meltdown in plants

Abstract

While most alien species fail to establish, some invade native communities and become widespread. Many of these communities have been invaded by multiple aliens, suggesting that aliens may cause invasional meltdowns. Here, we tested whether and how a third plant species affects the competitive outcome between alien and native plants through its soil legacy. We first conditioned soil with one of ten species (six natives and four aliens) or without plants. Then, we grew on these 11 soils, five aliens and five natives without competition, and with intra- or interspecific competition (all pairwise alien-native combinations). We found that aliens were not more competitive than natives when grown on soil conditioned by other natives or on non-conditioned soil. However, aliens were more competitive than natives on soil conditioned by other aliens. Although soil conditioning rarely affected the strength of competition between later plants, soil conditioned by aliens changed the competitive outcomes by affecting growth of aliens less negatively than that of natives. Microbiome analysis confirmed this finding by showing that the soil-legacy effects of one species on later species were less negative when their fungal endophyte communities were less similar; and that fungal endophyte communities were less similar between two aliens than between aliens and natives. Our study suggests that coexistence between aliens and natives is less likely with more alien species. Such invasional meltdown is likely mediated by spill-over of fungal endophytes, some of which are pathogenic.