Author

W. J. Allen

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5 Biophysics· 5 Ecology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 8 Organism:bacteria (other)· 7 Research Focus:protein folding & dynamics· 6 Research Focus:cell signaling & trafficking· 5 Assay:proteomics· 3 Assay:fluorescence / live-cell microscopy· 2 Approach:biophysical modeling· 1 Research Focus:cell type annotation· 1

Frequent Co-Authors

I. Collinson· 10 shared D. W. Watkins· 5 shared R. A. Corey· 5 shared A. Politis· 2 shared E. Pyle· 2 shared G. C. Pereira· 2 shared M. S. Dillingham· 2 shared R. Tuma· 2 shared

Activity by Year

2017-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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10 shared papersBiophysics

I. Collinson

Mutual co-authors in cluster

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11 papers

Recent·Most cited

Energy landscape steering mediates dynamic coupling in ATP-driven protein translocation by the bacterial Sec machinery

J. A. Crossley, W. J. Allen, D. W. Watkins, T. Sabir, S. E. Radford, R. Tuma, I. Collinson, T. Fessl

This study uses single-molecule fluorescence to show that SecYEG dynamically couples with SecA through energy landscape steering, enhancing protein translocation efficiency.

bioRxiv · Jun 6, 2023 · PreprintBiophysics

Rate-limiting transport of positive charges through the Sec-machinery is integral to the mechanism of protein transport

W. J. Allen, R. A. Corey, D. W. Watkins, A. S. F. Oliveira, K. Hards, G. M. Cook, I. Collinson

The paper investigates protein transport through the Sec-machinery, finding that arginine diffusion limits transport and PMF contributes via a proton ratchet mechanism.

bioRxiv · Feb 2, 2022Biochemistry

Towards a molecular mechanism underlying mitochondrial protein import through the TOM and TIM23 complexes

H. C. Ford, W. J. Allen, G. C. Pereira, X. Liu, M. S. Dillingham, I. Collinson

This study uses a split NanoLuc luciferase assay to analyze the kinetics of mitochondrial protein import through the TIM23MOTOR complex, identifying rate-limiting steps and their dependence on precursor properties.

bioRxiv · Aug 30, 2021Biochemistry

Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis

S. Alvira-de-Celis, D. W. Watkins, L. Troman, W. J. Allen, J. Lorriman, G. Degliesposti, E. J. Cohen, M. J. Beeby, B. Daum, V. A. M. Gold, J. M. Skehel, I. Collinson

The paper investigates the inter-membrane association between Sec and BAM translocons in bacterial outer-membrane biogenesis, showing their interaction ensures efficient OMP maturation.

bioRxiv · Jul 2, 2020Biochemistry

Refined measurement of SecA-driven protein transport reveals indirect coupling to ATP turnover

W. J. Allen, D. W. Watkins, M. S. Dillingham, I. Collinson

This study uses a split luciferase assay to measure SecA-driven protein transport in bacteria, revealing stepwise transport coupled to ATP hydrolysis without a distinct initiation event.

bioRxiv · May 8, 2020 · PreprintBiochemistry

HDX-MS reveals nucleotide-regulated, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon

Z. Ahdash, E. Pyle, W. J. Allen, R. A. Corey, I. Collinson, A. Politis

This study uses HDX-MS to show that ATP binding causes SecY to open and SecA to close, while ATP hydrolysis reverses this, supporting a Brownian ratchet mechanism for protein translocation.

bioRxiv · Apr 1, 2019Biophysics

A High-Resolution Luminescent Assay For Rapid And Continuous Monitoring Of Protein Translocation Across Biological Membranes

G. C. Pereira, W. J. Allen, D. W. Watkins, L. Buddrus, D. Noone, X. Liu, A. P. Richardson, A. Chacinska, I. Collinson

This paper introduces a high-resolution luminescent assay for rapid and continuous monitoring of protein translocation, demonstrating its application in bacterial and mitochondrial systems.

bioRxiv · Nov 1, 2018Biochemistry

Dynamic action of the Sec machinery during initiation, protein translocation and termination revealed by single molecule fluorescence

T. Fessl, D. Watkins, P. Oatley, W. J. Allen, R. A. Corey, J. Horne, S. A. Baldwin, S. E. Radford, I. Collinson, R. Tuma

This study uses single molecule FRET to analyze Sec machinery dynamics during protein translocation, revealing initiation, transport, and termination phases with specific roles for SecA and ATP hydrolysis.

bioRxiv · Jan 17, 2018Biophysics

Specific cardiolipin-SecY interactions are required for proton-motive-force stimulation of protein secretion

R. A. Corey, E. Pyle, W. J. Allen, M. Casiraghi, B. Miroux, I. Arechaga, A. Politis, I. Collinson

This study identifies and validates two conserved cardiolipin binding sites on the SecA-SecYEG complex, showing their role in ATP and PMF-driven protein secretion.

bioRxiv · Dec 6, 2017Biophysics

ATP-induced asymmetric pre-protein folding: a driver of protein translocation?

R. A. Corey, W. J. Allen, I. Collinson

The paper uses molecular dynamics simulations to study ATP-driven pre-protein translocation through the SecA-SecYEG complex, showing nucleotide-dependent asymmetry in secondary structure formation.

bioRxiv · Dec 6, 2017Biophysics

Intraspecific variation in indirect plant-soil feedbacks as a driver of a wetland plant invasion

W. J. Allen, L. A. Meyerson, A. J. Flick, J. T. Cronin

This study investigates how intraspecific variation in plant-soil feedbacks interacts with environmental factors to influence competition between native and invasive reed lineages.

bioRxiv · Jul 7, 2017Ecology