Influence of Sodium Salicylate on Adventitious Organogenesis of a Commercial Cucumber Cultivar

Abstract

The effect of different concentrations of sodium salicylate (NaSA), a phenolic compound, on in vitro callus and shoot regeneration of cucumber (Cucumis sativus L.) was investigated. Four-day-old cotyledon explants from the Spanish cultivar Marketer were employed. MS-derived shoot induction medium containing 0.5 mg L-1 IAA and 2.5 mg L-1 BAP was supplemented with NaSA. Frequency and shoot number were enhanced by 1.5-fold with NaSA at 0.1 M. Higher salicylate levels led to increased callus formation and decreased shoot regeneration. The application of sodium salicylate at a specific concentration showed a positive trend in in vitro adventitious organogenesis of a commercial cucumber cultivar. Some probable mechanisms that may underlie the beneficial effects of salicylic acid/salicylates on in vitro regeneration were also discussed.