organoid / primary culture

This paper develops a metastasis-on-a-chip model to study thyroid cancer to lung metastasis, showing that RCAN1.4 suppression enhances metastasis and that NK-92 cells reduce metastases.

The paper investigates how EGF and EREG influence early oncogenic transformation in intestinal organoids by altering crypt and villus development.

This study shows that targeting REV1 in colorectal cancer enhances chemotherapy sensitivity in a context-dependent manner and inhibits organoid growth.

This study identifies MAST1 as a neuron-specific regulator of mitochondrial trafficking and abundance, linking its mutations to neuronal mitochondrial dysfunction and microcephaly.

This study identifies cholinergic and adrenergic neural signals that activate human intestinal L-cells, triggering hormone secretion and calcium waves.

This paper introduces a microfluidic platform for applying multiaxial mechanical loading to cell-laden scaffolds, demonstrating its ability to promote cardiac tissue organization and contractile activity.

This paper develops a method to print collagen-I scaffolds directly inside organ-on-chip devices, enabling perfusable and stretchable mammary tissue models with compartmentalized fluidic control.

This paper shows that adjusting Activin A levels in a 3D bioreactor system can generate liver-like structures from human stem cells, mimicking early liver development.

This paper introduces a method to rapidly create customized, autoclavable cell culture accessories using polycarbonate thermoforming from 3D printed molds, demonstrating compatibility with human iPSC culture and differentiation.

This study develops a 3D human bone-mimetic niche system to investigate how different bone compartments regulate breast cancer cell quiescence and therapy response.

This paper introduces a method to generate oviductal assembloids from mouse and human tissues to study oviduct function and embryo-maternal interactions in vitro.

This study shows that the order of RSV and RV infection in infants determines disease outcome through type III interferon-mediated viral interference.

This study develops human spinal cord organoids and assembloids to model oligodendrocyte development and disease states, showing they recapitulate developmental signatures and cytokine-induced dysfunction seen in multiple sclerosis.

This paper introduces tunable fibrous hydrogels to study how specific ligands direct different cell migration modes in 3D environments.

This study shows that vascular network formation by hiPSC-derived endothelial progenitors in 3D hydrogels depends on matrix stiffness, contractility, and cell-induced remodeling, not just mechanotransduction.

This paper introduces a method using PEG microgels to enable reproducible, scalable iPSC organoid production and differentiation into specific tissues.

This paper develops a lab-on-a-chip system integrating a human BBB model with neural organoids to study BBB function and nanoparticle transport, showing BBB integrity and nanoparticle entry into neural tissue.

This study shows that high glucose in kidney organoids causes inflammation and epithelial detachment, which can be reversed by targeting cytokines and signaling pathways.

The paper introduces Microdomes, a 3D biology platform that enables shear-free handling and full-spectrum optical imaging of spheroids and organoids.

This study models the MTARC1 A165T variant in human liver organoids, showing reduced steatosis, inflammation, and fibrosis compared to reference sequence organoids.

This paper introduces Brainobot, a biohybrid robotic system using brain organoid reservoir computing to enable real-world decision-making tasks like object grasping and laser chasing with high efficiency and low energy consumption.

This study investigates how scaffold architecture influences cell-scaffold interactions, showing that scaffold dimensionality and fiber spacing control the transition from quasi-2D to 3D cellular behavior.

The paper identifies 9S-HODE, a lipid secreted by lean adipocytes, as a ferroptosis inducer that suppresses breast cancer while sparing normal cells, with therapeutic potential in obese mice.

This paper evaluates two cryopreservation strategies for amniotic fluid cells to improve organoid derivation efficiency and identifies an optimal freezing medium.

This study identifies SLAP as a tumor suppressor that limits SFK activity in colonic epithelial cells, preventing tumorigenesis and hyperproliferation.

This study induces skeletal muscle, neuronal, and endothelial cells from bovine embryonic stem cells and demonstrates their self-organization into vascularized tissue structures.

This study introduces proxiloids, a scalable human-induced pluripotent stem cell differentiation method that models kidney proximal tubule toxicity more accurately than existing methods.

This paper develops a biophysical platform to deliver electromechanical stimulation to engineered cardiac tissues and shows that synchronized stimulation increases contractile force.

This study develops an iPSC-derived urothelial organoid model to investigate how hormones affect host defense against urinary tract infections.