Magnesium maintains length of circadian period in Arabidopsis thaliana

Abstract

The circadian clock coordinates the physiological response of a biological system to day and night rhythms through complex loops of transcriptional/ translational regulation. It can respond to external stimuli and adjust generated circadian oscillations accordingly to keep an endogenous period close to 24 h. To date, the interaction between nutritional status and circadian rhythms in plants is poorly understood. Magnesium (Mg) is essential for numerous biological processes in plants and its homeostasis is crucial to maintain optimal development and growth. Magnesium deficiency in young Arabidopsis thaliana seedlings increased the circadian period of pCCA1:LUC oscillations and dampened its amplitude in constant light in a dose-dependent manner. Although circadian period increase by Mg deficiency was light dependent, it did not depend on active photosynthesis. Mathematical modelling of the Mg input to the circadian clock reproduced the experimental increase of the circadian period and suggested that Mg is likely to affect global transcription/translation levels rather than a single component of the circadian oscillator. The model prediction was supported by a synergistic interaction between Mg deficiency and cyclohexamide, an inhibitor of translation. These findings suggest that proper Mg supply is required to support proper timekeeping in plants. One sentence summaryMagnesium maintains the circadian period in Arabidopsis seedlings and interferes with the circadian oscillator most likely through translational mechanisms.