A Trizol-Beadbeater RNA extraction method for yeast, and comparison to Novogene RNA extraction

Abstract

58 samples of S. cerevisiae were sent to the Novogene Corporation for the intended purpose of RNA extraction, cDNA preparation, and sequencing. RNA was extracted by Novogene. However, yields of total RNA were about 1%, perhaps 1.25%, of the theoretically expected amount, which seemed low. Therefore, we extracted RNA locally using Trizol and bead-beating from a second, identical aliquot of 55 of the samples sent to Novogene. This local extraction gave yields that were on average 40% of the theoretical expectation, about 40 fold higher than the yields obtained by Novogene.