A comprehensive personal omics clinical interpreter based on genomic and transcriptomic profiles

Abstract

Advances in precision medicine rely on the accurate identification and analysis of molecular alterations for personalized diagnostic, prognostic, and therapeutic decision-making. A critical obstacle is the integration of heterogeneous interpretations of clinically actionable alterations from various knowledgebases. Here, we present the Personal Omics Interpreter (POI), a web-based application engineered to aggregate and interpret therapeutic options, including targeted, immunological, and chemotherapeutic agents, by leveraging personal genomic and transcriptomic profiles. POI employs the Precision Medicine Knowledgebase (PreMedKB), an updated harmonized resource we previously reported, to annotate the clinically actionable somatic variants. It further incorporates a predictive algorithm to broaden therapeutic options according to established gene-gene interactions and offers insights into phenotypic responses of chemotherapeutic agents through phasing germline diplotypes. Validated against three cohort datasets encompassing over 22,000 cancer patients, POI demonstrates consistently high matching rates (94.7 [~] 95.6%) between patients and suggested therapies, highlighting its potential in supporting precision-driven informed treatment strategies.