LysM Receptor Proteins are Required for Ectomycorrhizal Symbiosis in Poplar

Abstract

Successful symbiotic associations often rely on the perception of specific microbial signals by host plants through pattern recognition receptors. LysM receptor-like kinases are key receptors in both rhizobial and arbuscular mycorrhizal symbioses. In this study, we provide the first evidence that such LysM receptor-like kinases also control the establishment of ectomycorrhizal associations in poplar. Using CRISPR/Cas9-mediated genome editing, we knocked out three NFP-like candidate genes in various combinations. We examined their roles in the perception of the ectomycorrhizal fungus Laccaria bicolor, as well as chitin oligomers and lipo-chitooligosaccharides. We demonstrate that all three receptors are necessary for the recognition of symbiosis-related signals, calcium spiking, symbiotic gene expression, and ectomycorrhizal root phenotypes.