Development of additional microsatellite primers for the mangrove tree species Avicennia germinans

Abstract

ObjectiveThe objective of this study was to develop additional microsatellite primers for the mangrove tree species Avicennia germinans that work in multiplex PCR panels to enable cost effective population analyses of this species at a finer scale. ResultsPrimer sets were identified from whole genome sequencing data and combined into multiplex PCR panels. Five multiplex reactions containing 20 novel primer sets with trinucleotide repeats were successfully developed. Fifteen of the microsatellite loci were polymorphic in the samples tested, with 1-4 alleles per locus.