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Plant BiologybioRxiv
Preprint

Heterogenous transcriptomes of Nicotiana tabacum BY-2 suspension cell lines adapted to various osmoticum

Tomasz SkrzypczakiD, Przemysław WojtaszekiD, Anna Kasprowicz‐MaluśkiiD

Abstract

Plants abiotic stress response and adaptation belong to the most important subjects in plants biology. Here, we present Nicotiana tabacum suspension cell lines adapted during long term cultures to high concentrations of NaCl, KCl, mannitol and sorbitol. Obtained lines differ in osmotic stress agents and final media osmolarities. RNA-seq analysis revealed similarities, as well as differences in adapted lines transcriptomes. Presented here BY-2 cells lines form a good model to reveal molecular mechanisms of plants adaptations to salt and osmotic stress on cellular level.

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