Molecular evidence that barnacle attachment may be highly associated with a few eukaryotes in the marine biofilm

A preliminary study on the effects of different antifouling coatings on the colonization of barnacles by investigating the diversity parameters of microflora in the coatings has been conducted. In this study, twenty-five different carbon nanotube materials were used to prepare nanocoating paints with a concentration of 0.2% w/v in iron red matrix and immersed in the sea fields for about two weeks. The number of colonized barnacles on each coating film was counted, and high-throughput sequencing analysis of their corresponding biofilm samples was performed to investigate the composition of prokaryotes and eukaryotes. It was discovered that there is no significant difference in the composition of prokaryotes in biofilms on coatings with distinct colonization levels of barnacles, but there is a significant difference in the composition of some eukaryotes such as Choreotrichia and Cryptomonadales.