Influence of ocean warming and acidification on juveniles of the true giant clam, Tridacna gigas, and its microalgal symbionts

Abstract

Uncontrolled carbon dioxide emissions from human activities contribute to ocean warming and acidification. These alterations in ocean chemistry threaten marine organisms, such as the true giant clam, Tridacna gigas, which is already imperiled due to overharvesting and habitat destruction. To gain an understanding of the physiological and molecular responses of T. gigas and its symbiotic dinoflagellates to ocean warming and acidification, we subjected juvenile individuals to different treatments simulating predicted seawater pH (7.6 and 8.0) and temperature (28{degrees}C, 30{degrees}C, 32{degrees}C and 34{degrees}C) levels for the next century. Juvenile giant clams were able to tolerate sustained exposure to temperatures of up to 32{degrees}C and pH as low as 7.6, while exposure to higher temperature (34{degrees}C), regardless of pH level, resulted in total mortality after a week. However, symbiosis was compromised even in the sublethal treatments, as indicated by the decrease in Symbiodiniaceae density and changes in symbiont gene expression. Symbionts significantly upregulated genes involved in splicing, translation, fatty acid metabolism, and DNA repair, which may constitute an adaptive response, while downregulating genes involved in photosynthesis and transmembrane transport, suggests impaired transfer of photosynthates to the host. These findings demonstrate the vulnerability of the juvenile T. gigas holobiont to heat stress, highlighting the critical importance of continued conservation and management alongside efforts to mitigate global changes in ocean conditions to safeguard this iconic marine bivalve. Summary StatementThis study investigates physiological and molecular responses of Tridacna gigas to seawater warming and acidification, providing insights into the potential future of endangered giant clam populations in a changing ocean.