GEMBS -- high through-put processing for DNA methylation data from Whole Genome Bisulfite Sequencing (WGBS)

INTRODUCTION INTRODUCTION RESULTS CONCLUSIONS Sample data Benchmark Variant calling from WGBS Genotype calling MATERIAL and METHODS References DNA methylation is essential for normal embryogenesis and development in mammals and most commonly found at CpG dinucleotides. Whole genome sequencing of bisulfite converted DNA (WGBS) currently represents the gold standard for studying DNA methylation at genomic level as contrary to other techniques, it provides an unbiased view of the entire genome at single base pair resolution (see Bock et al. 2012). In practice, due to its comparatively high cost, its application for the analysis of large data sets (i.e. > 50 samples) has been lagging behind other more cost-efficient platforms, s ...