Nelly N. Olova

Increased stress in early life epigenetically rewires pathways related to aging and longevity

This study shows that early-life stress accelerates epigenetic aging in zebra finches by altering DNA methylation and metabolic pathways linked to aging.

Whole Genome Methylation Sequencing via Enzymatic Conversion (EM-seq): Protocol, Data Processing and Analysis

The paper introduces EM-seq, a new method for whole genome methylation sequencing that avoids the biases of WGBS by using enzymatic conversion, and demonstrates its advantages in accuracy and efficiency.

Region-Based Epigenetic Clock Design Improves RRBS-Based Age Prediction

This paper improves age prediction from RRBS data by using region-based methylation averages instead of individual CpGs, showing better performance on external datasets and low coverage data.

Conserved and divergent features of DNA methylation in embryonic stem cell-derived neurons

The paper examines DNA methylation patterns in neurons derived from mouse ES-cells and cultured primary neurons, finding similarities and differences in mCH deposition compared to in vivo conditions.

Partial reprogramming induces a steady decline in epigenetic age before loss of somatic identity

This study shows that partial reprogramming reduces epigenetic age in human cells but decouples it from the loss of somatic identity, suggesting a safe window for rejuvenation without teratoma risk.

Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data

This study compares WGBS library preparation methods and identifies biases in sequencing data, finding that bisulfite conversion and PCR amplification are major sources of bias.

Tracking the embryonic stem cell transition from ground state pluripotency

The paper tracks the transition of mouse embryonic stem cells from ground state pluripotency to a formative phase, showing acute exit from naive status and transcriptome changes similar to early post-implantation epiblast.