Genome assembly and annotations of a Cyprinidae fish, Sinibrama wui

Abstract

Sinibrama wui is a freshwater fish belonging to the Cyprinidae family, primarily found in the middle and upper Yangtze River of China. However, no reference genome sequence for S. wui has yet to be established. The present study presents a high-quality, chromosome-level genome assembly of S. wui through PacBio Biosciences sequencing and Hi-C technologies. The genome size was 1028.95 Mb, with a contig N50 length of 37.21 Mb and scaffold N50 of 40.74 Mb. We assembled 124 contigs, within which 68 were organized into 24 chromosomes. In total, 25,967 protein-coding genes were predicted, with 99.10% functionally annotated. We further analyzed the evolutionary relationships, gene families expansion, and positive selection of S. wui. Our data showed that S. wui diverged from Megalobrama amblycephala approximately 10.1 million years ago, and the expanded gene families had enriched in the lipid metabolism and nervous system. These datasets offer valuable genomic resources for future investigations studies on the genetic conservation, selective breeding, and evolution of S. wui.