Transcriptome based changes in expression of detoxification genes of Bemisia tabaci under destruxin A treatment

Abstract

BackgroundBemisia tabaci is an important agricultural pest that has been causing significant economic losses to crops across the globe. Destruxins are secondary metabolites of entomopathogenic fungi which can be used as a potential biopesticide against B. tabaci. However, little is known about the molecular mechanism regulating the defense response of B. tabaci post destruxin application. ResultsIn this study, we explored the molecular responses of B. tabaci exposed to destruxin A (DA) using RNA-Seq and differentially expressed gene (DEG) analysis. A total of 1702, 616, and 555 DEGs were identified in B. tabaci after 4, 8, and 12 h of destruxin A treatment. In addition, 40 putative detoxification-related DEGs, including 29 cytochrome P450s (P450s), 5 glutathione S-transferases (GSTs), and 5 carboxylesterases (CarEs) were also identified. Quantitative real-time PCR analysis indicated that the expression profiles of 19 random DEGs were consistent with the RNA-Seq results. ConclusionThese findings serve as valuable information for a better understanding of the interaction and molecular mechanisms involved in the defense response of B. tabaci against DA.