Insect Resistance and Genetics

Investigation of V1016G kdr Mutation during Pyrethroid Insecticide Resistance in Aedes aegypti from Nagapattinam district of Tamil Nadu, India

The paper investigates the V1016G kdr mutation in Aedes aegypti from Tamil Nadu, India, finding heterozygous mutation prevalence linked to pyrethroid resistance.

Identification of insecticidal double-stranded RNAs targeting the proteasome pathway in the hop flea beetle Psylliodes attenuata using dsRIP

This study identifies insecticidal dsRNAs targeting the proteasome pathway in the hop flea beetle using dsRIP and demonstrates their efficacy in bioassays.

A shared molecular target but distinct genetic routes to diamide resistance in Helicoverpa armigera (Lepidoptera: Noctuidae)

The paper investigates resistance to diamide insecticides in Helicoverpa armigera, finding distinct genetic routes for chlorantraniliprole and flubendiamide resistance despite sharing the same molecular target.

Metabolic compensation via gluconeogenesis explains the non-essentiality of glycogen phosphorylase as an insecticidal target in Plutella xylostella

The paper investigates whether glycogen phosphorylase (GP) can be an insecticidal target in Plutella xylostella by evaluating the effects of GP inhibitors and showing that metabolic compensation via gluconeogenesis prevents insecticidal activity.

Context and exposure history shape insecticide sensitivity in Drosophila melanogaster

This study shows insecticide sensitivity in Drosophila varies with developmental stage, sex, temperature, and prior exposure, revealing context-dependent toxicity and potential trade-offs in stress resilience.

Insecticide resistance of Culex pipiens f. pipiens is geographically widespread in Germany and displays an unmatched genotypic and phenotypic resistance state

The paper assesses insecticide resistance in Culex pipiens f. pipiens across Germany, finding high kdr allele frequencies and phenotypic resistance in urban areas.

High-throughput phenotypic profiling of insecticide responses in mosquito larvae

This study develops high-throughput assays to profile insecticide responses in mosquito larvae, testing compounds and establishing baseline dose-response curves.

Intersecting experimental evolution and CRISPR screens to identify novel toxin resistance loci

The paper identifies genes involved in octanoic acid resistance by combining experimental evolution and CRISPR screens in Drosophila species.

Global changes in alternative splicing are associated with insecticide response and resistance

This study shows that insecticide-resistant Drosophila suzukii exhibits increased global alternative splicing events, enriched in stress response and resistance processes, even without substantial differential gene expression.

Evaluating pesticide mixtures for resistance management in asexual insect pests

This study evaluates pesticide mixtures for resistance management in asexual insect pests and finds they can delay resistance evolution compared to sexual populations.

Novel ddPCR diagnostic assays for the high-throughput surveillance of Kdr mutations in Aedes albopictus

The paper develops ddPCR assays for high-throughput Kdr mutation surveillance in Aedes albopictus, showing high accuracy and low mutation prevalence in French populations.

Molecular and functional characterization of CYP6AN1 orthologs involved in capsaicin metabolism in two Helicoverpa species

This study identifies and compares CYP6AN1 orthologs in two Helicoverpa species, showing differences in expression, regulation, and substrate metabolism of capsaicin.

Paradigm shift for cry gene expression in Bacillus thuringiensis

This paper shows that VipR regulates insecticidal gene expression in Bt strains independently of sporulation, leading to early toxin production.

Synergism of Cry1Aa protein against Grapholita molesta (Busck) by a cadherin fragment from Spodoptera exigua (Hübner)

This study shows that a cadherin fragment from Spodoptera exigua enhances Cry1Aa toxicity against Grapholita molesta but not S. exigua, likely through increased binding to midgut receptors.

Reversion of pyrethroid resistant phenotypes in Aphis glycines by topical delivery of dsRNA targeting resistance alleles at the vgsc locus

This study shows that applying dsRNAs targeting vgsc-h1 genes reduces mortality and reproduction in pyrethroid-resistant soybean aphids, enhancing insecticide effectiveness.

Transposable element disruption of a second thyroglobulin-like gene confers Vip3Aa resistance in Helicoverpa armigera

The paper identifies a transposable element insertion in a second thyroglobulin-like gene as a mechanism of Vip3Aa resistance in Helicoverpa armigera.

Tracking kdr Alleles Associated with Pyrethroid Resistance in Aedes albopictus across Italy: A Nationwide Genotypic Dataset by MosqIRIT Network.

This paper presents a georeferenced dataset of pyrethroid resistance mutations in Aedes albopictus across Italy, supporting resistance monitoring and ecological modeling.

Integrated risk assessment reveals low biological activity of RNA-based crop protection formulations across plant and non-target systems

The paper assesses the biological activity of RNA-based crop protection formulations in plants and non-target organisms, finding low activity across tested systems.

Transcriptomic profiling reveals multiple mechanisms of insecticide resistance in Aedes aegypti from Angola

This study identifies multiple insecticide resistance mechanisms in Angolan Aedes aegypti using transcriptomic analysis, revealing shared and novel resistance genes compared to other regions.

A horizontally transferred fungal deubiquitinase facilitates insecticides resistance in whitefly

This study identifies a horizontally transferred fungal deubiquitinase, BtUCH19, that enhances insecticide resistance in whiteflies by stabilizing a detoxification enzyme through ubiquitin modification.

A tissue-resolved transcriptomic atlas of adult male Halyomorpha halys reveals tissue-specific RNAi machinery and a minimal systemic response to non-specific dsRNA

Creating resistance to the whitefly Bemisia tabaci in cassava through RNAi-mediated targeting of multiple insect metabolic processes

This study develops transgenic cassava plants using RNAi to target whitefly metabolic processes, achieving high insecticidal activity and potential for field control of whitefly populations in East Africa.

Unlocking the (Coleoptera: Tenebrionidae)-Combatting Capabilities of INTA Mo4-4 through genomic and phenotypic characterization

This study characterizes Bacillus thuringiensis INTA Mo4-4, showing it is more toxic to Alphitobius diaperinus than DSM-2803 and encodes pesticidal proteins.

Chromosomal markerless integration of anthelmintic Cry proteins into the Bacillus thuringiensis genome

This paper develops markerless integration methods for Cry proteins in Bt to enable safe and stable Cry expression for anthelmintic applications.

Study of the molecular nature of resistance to bifenazate in a Tetranychus urticae Koch Laboratory Strain

This study identifies the G126S mutation in Tetranychus urticae as a key driver of bifenazate resistance, showing its rapid fixation under selection and its sufficiency for high-level resistance in a Russian population.

Resistance to Pyrethroids in Aedes aegypti: Insights into Transcriptomic Response to Different Insecticide Concentrations Transcriptomic responses of Aedes aegypti to insecticide concentrations

This study investigates transcriptomic responses of Aedes aegypti to different concentrations of permethrin and lambda-cyhalothrin, revealing distinct resistance mechanisms and concentration-dependent gene expression patterns.

Orally Delivered dsRNA-Derived siRNAs Reach the Central Nervous System in Leptinotarsa decemlineata

This study shows orally ingested dsRNA reaches the CNS of Leptinotarsa decemlineata and is processed into siRNAs, providing evidence of systemic RNAi transport in this pest.

Dietary landscapes shape genotype- and sex-specific responses to insecticides

The paper shows how a resistance allele affects reproductive traits differently in male and female fruit flies depending on diet and insecticide exposure.

The collision of two genomes threatens global food security

The paper studies hybridization between native and invasive Helicoverpa moths in Brazil, showing rapid adaptive introgression of pesticide resistance genes with implications for global food security.

Structural and Evolutionary Constraints of Organophosphate Resistance in Dipteran Carboxylesterases

This study investigates the molecular basis and evolutionary constraints of organophosphate resistance in dipteran carboxylesterases, revealing sequence context-dependent success of a mutation and trade-offs between affinity and turnover.