Characterization and identification of enzyme-producing microflora isolated from the gut of freshwater Prawn, Macrobrachium Spp

Abstract

Isolation of extracellular enzyme degrading aerobic bacteria in the digestive tracts of fresh water prawn was carried out. Gut bacteria were isolated on carboxymethyl cellulose agar plates, starch agar media plates, gelatine peptone agar media plates. The isolated strain was qualitatively screen based on their extracellular enzyme on selective media. The strain was characterised based on morphological, physiological and biochemical characterization identified Bacillus species. Bacillus species was isolated by colony characterization carried out by using Gelatin agar Media, carboxymethyl cellulose media and congo red CMC media and starch agar media for different enzyms. Isolates are capable of hydrolysing proteins and carbohydrates indicating their importance in fish nutrition.