Isolation and Identification of P(3HB)-Degrading Bacteria from Malaysian Environment

The aim of this study was to identify Poly(3-hydroxybutyrate) [P(3HB)] degrading bacterial strains in Penang Malaysia. Samples were collected from various locations in Penang. Five isolates (SMA-1, SMB-2, SMC-3, SMD-4, and SME-5) with highest P(3HB) degradation indices were isolated through meticulous screening. These gram-negative bacilli were characterized first morphologically and then through scanning electron microscopy. Growth profiles of the isolates revealed that at 24 hrs, SME-5 showed the highest growth rate. Maximum depolymerase enzyme activity for all five isolates was observed after 72 hrs. 16S rDNA sequencing identified all isolates as Comamonas testosteroni. This study provides insights into the growth characteristics and extracellular depolymerase activity of P(3HB)-degrading bacteria isolated from the Malaysian environment. It highlights the potential of Comamonas testosteroni strains as P(3HB) bioplastic degraders in diverse habitats.