Constitutive and regulatory responses of Arabidopsis thaliana to harmonically oscillating light

Abstract

The rate of net CO2 uptake is proportional to dim light and saturates when the light exceeds the plant assimilation capacity. This simple relationship between constant light and photosynthesis becomes intriguingly complex when the light oscillates. The rates of photosynthesis may differ between the descending and ascending phases of light oscillation. This hysteresis changes with the frequency and amplitude of the light and reports on the dynamics of the photosynthetic reactions and their regulation. Here, we investigated the chlorophyll fluorescence response of Arabidopsis thaliana to light oscillating with three different amplitudes: 100-200, 100-400, and 100-800 mol photons{middle dot}m-2{middle dot}s-1, each with periods ranging from 1 s to 8 min. The light amplitudes and periods were chosen to represent light patterns often appearing in nature. Three genotypes were compared: wild-type Col-0 and npq1 and npq4 mutants that are incapacitated in the rapidly reversible energy-dependent non-photochemical quenching (qE). The experiments identified two major dynamic patterns. One was found in oscillation periods shorter than 30 s, characterized by constitutive non-linearity and hysteresis. The other was mainly formed by regulatory non-linearity and hysteresis, occurring when the oscillation periods were longer than 30 s. The mathematical model simulating the chlorophyll fluorescence dynamics qualitatively reproduced the constitutive and regulatory dynamic patterns observed in the experiments. The model simulations illustrated the dynamics of non-photochemical quencher activation, plastoquinone pool reduction, and lumen pH that form the constitutive and regulatory non-linearities. The model simulations provided mechanistic insight into molecular processes forming the plant response to oscillating light.