Nitrogen dynamics and yield performance of an elite bread wheat line with BNI capacity expressed in an alkaline soil

Abstract

Wheat with biological nitrification inhibition (BNI) has been shown to be effective in acidic soils. BNI expressions in alkaline soil have not been documented in field studies. We present the first observation of BNI effects in alkaline soil trials (pH 8.6-8.7) of elite spring wheat that carries the Lr#N short arm from Leymus racemosus. BNI was expressed as lower soil nitrate in three trials conducted in an irrigated wheat system in northwestern Mexico. In one year, we observed 24-37% lower nitrate in soil of the translocation line compared to the control after the second ammonium-N fertilizer application. Lower nitrate was observed in the row and in the furrow. The largest difference between the control and the translocation line was approximately one month after the second ammonium-N split application (73-77% less nitrate). Potential nitrification rates were 27-32% lower in soil from the translocation line compared to the control, one week after a high ammonium-N application. Higher flag leaf nitrate concentration of the translocation line could be related to the strongly reduced soil nitrate. The translocation line took longer to reach anthesis and flowering. In two experiments, the translocation line was equivalent in terms of grain yield, whereas in one experiment it produced lower yields, with fewer grains per spike and reduced number of spikes per area. Protein content and grain N uptake of the translocation line were similar to the control. We conclude that BNI reduces nitrification with a spatially and temporally significant impact under alkaline soil and high N input conditions. This finding points to the potential major environmental benefits that could be achieved also in non-acidic soil spring wheat systems worldwide. The effect of the Lr#N short arm on yield quantity and quality in other elite lines needs further investigation.