Dynamic Disorder in Chlorophyll Aggregation and Light-Harvesting Complex II in the Plant Thylakoid Membrane using Coarse-Grained Simulations

The dynamics of the aggregated light-harvesting complex (LHCII) associated with its antennae pigments can be crucial for a transition between light harvesting and dissipative states pivotal for non-photochemical quenching (NPQ). To this end, aggregation of chlorophyll-a (CLA) without the LHCII and pigment binding LHCII monomers in the plant thylakoid membranes have been investigated using coarse-grained molecular dynamics simulations at 293 K. Both CLA without the LHCII and pigment-binding LHCII monomers dynamically form and break dimers and higher-order aggregates in thylakoids within the simulation time. The contact lifetime and waiting time distributions of CLA dimers exhibit multiple time scales including most populated fast time scales and less populated slow time scales. The survival probability of CLA dimer in the absence of the LHCII follows a non-exponential decay with multiple residence time scales, leading to a time-dependent rate, unlike conventional rate theory. Such non-exponential decay of survival manifests the emergence of dynamic disorder in CLA without the LHCII resulting from the coupling between time scales of dimer formation and higher-order aggregates. The conformational fluctuations of the LHCII known for inter-CLA coupling variation occur on multiple time scales comparable to the LHCII dimer residence time scales leading to less probable but comparable and more probable slower inter-CLA fluctuations. This indicates the dynamic coupling in the LHCII conformations and their aggregates with the antennae pigments can result in dynamic disorder which will be highly relevant for the light-harvesting efficiency and regulation of NPQ. TOC Graphic O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=111 SRC="FIGDIR/small/638782v1_ufig1.gif" ALT="Figure 1"> View larger version (57K): org.highwire.dtl.DTLVardef@5fd895org.highwire.dtl.DTLVardef@8456cforg.highwire.dtl.DTLVardef@5f5c69org.highwire.dtl.DTLVardef@abf6d0_HPS_FORMAT_FIGEXP M_FIG C_FIG