Resistance gene discovery and cloning by sequence capture and association genetics

Sanu Arora; Burkhard Steuernagel; Kumar Gaurav; Sutha Chandramohan; Yunming Long; Oadi Matny; Ryan Johnson; Jacob M. Enk; Sambasivam K. Periyannan; Narinder Singh; Muhammad Asyraf Md Hatta; Naveenkumar Athiyannan; Jitender Cheema; Guotai Yu; Ngonidzashe Kangara; Sreya Ghosh; Les J. Szabo; Jesse Poland; Harbans S. Bariana; Jonathan D. G. Jones; Alison R. Bentley; Mick Ayliffe; Eric L. Olson; Steven S. Xu; Brian Joel Steffenson; Evans S. Lagudah; Brande B. H. Wulff

doi:10.1038/s41587-018-0007-9

GenomicsNature BiotechnologyFeb 1, 2019

Peer-reviewed journal article

Resistance gene discovery and cloning by sequence capture and association genetics

Sanu Arora iD, Burkhard Steuernagel iD, Kumar Gaurav, Sutha Chandramohan, Yunming Long, Oadi Matny iD, Ryan Johnson, Jacob M. Enk iD, Sambasivam K. Periyannan iD, Narinder Singh iD, Muhammad Asyraf Md Hatta iD, Naveenkumar Athiyannan iD, Jitender Cheema iD, Guotai Yu iD, Ngonidzashe Kangara iD, Sreya Ghosh iD, Les J. Szabo iD, Jesse Poland iD, Harbans S. Bariana iD, Jonathan D. G. Jones iD, Alison R. Bentley iD, Mick Ayliffe, Eric L. Olson iD, Steven S. Xu iD, Brian Joel Steffenson iD, , Brande B. H. Wulff iD

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Abstract

Genetic resistance is the most economic and environmentally sustainable approach for crop disease protection. Disease resistance (R) genes from wild relatives are a valuable resource for breeding resistant crops. However, introgression of R genes into crops is a lengthy process often associated with co-integration of deleterious linked genes1, 2 and pathogens can rapidly evolve to overcome R genes when deployed singly3. Introducing multiple cloned R genes into crops as a stack would avoid linkage drag and delay emergence of resistance-breaking pathogen races4. However, current R gene cloning methods require segregating or mutant progenies5-10, which are difficult to generate for many wild relatives due to poor agronomic traits. We exploited natural pan-genome variation in a wild diploid wheat by combining association genetics with R gene enrichment sequencing (AgRenSeq) to clone four stem rust resistance genes in <6 months. RenSeq combined with diversity panels is therefore a major advance in isolating R genes for engineering broad-spectrum resistance in crops.