Landrace and bred accessions of allotetraploid sour cherry (Prunus cerasus L.) reveal variation in subgenome dosage and subgenome expression bias

Abstract

Subgenome dominance is a phenomenon observed in many allopolyploids where one parental genome exhibits stronger influence over phenotype than the other parental genomes. This may present as preferential retention of one subgenome through fractionation, replacement via homoeologous exchange, or as subgenome expression bias, where one subgenome is expressed at a higher abundance compared to other subgenomes. Sour cherry (Prunus cerasus) is an allotetraploid fruit tree species resulting from an interspecific cross between extant relatives of ground cherry (P. fruticosa) and sweet cherry (P. avium). Prior comparative genomic analyses suggest that the sour cherry cultivar Montmorency contains three subgenomes. Subgenomes A and A, each present in one copy, are derived from a P. fruticosa-like ancestor, and B, present in two copies, is derived from a P. avium-like ancestor. In this study we investigated the subgenome dynamics of the three subgenomes of sour cherry in four diverse landraces and two cultivars, including Montmorency. We found evidence of 26 homoeologous exchange events and five whole-homoeolog replacements relative to Montmorency in three of the six accessions. We also detected subgenome expression bias favoring the A and A subgenomes over the B subgenome, the magnitude of which differs between accessions and changes over the course of fruit development. Lastly, we show differences in dosage variation and expression bias of four previously-described genes in Montmorency associated with fruit softening, a key trait in this crop. These findings on subgenome dominance offer valuable insights into how this phenomenon may influence traits important for sour cherry breeding.