Chemical Crosstalk between the Common Soil Bacteria Pseudomonas chlororaphis and Bacillus subtilis

Abstract

Chemical conversations between soil inhabitants are of tremendous importance to the health of many ecosystems, and at the same time detailed molecular knowledge underlying these conversations is surprisingly scarce. One of the major bacterial genera inhabiting the rhizosphere is Pseudomonas, of which most species are known to produce phenazines, which carry antibiotic properties. Pseudomonas chlororaphis, a common rhizosphere dwelling species with plant growth-promoting traits, produces phenazine-1-carboxamide (PCN). This study examines how the production of PCN by Pseudomonas affects another common species in soil that it often encounters, namely Bacillus subtilis. When both species were cultured at close distance, distinct and visible changes in colony morphologies were observed with-out changes in growth rates. Interestingly, a clear transformation occurred in the morphology of B. subtilis colonies in the presence of supplemented PCN, indicating the role of phenazines in affecting colony morphology. In addition, untargeted metabolomics analyses showed a decrease in the production of plipastatin and surfactin by B. subtilis in the presence of P. chlororaphis. Our results indicate that PCN induces changes in morphology and signaling of B. subtilis without significantly affecting its growth. We hypothesize that P. chlororaphis and B. subtilis sense one another and act to conserve energy while avoiding competition.