Social rank-order stability of mice revealed by a novel food competition paradigm in combination with available space competition paradigms

Abstract

Psychological, behavioral and biological studies on social organization and competition with animal models are boosting. The mouse has been recognized as a valuable and economic model animal for biomedical research in social behaviors, however, currently available food competition paradigms for mice remain limited. Discrepant paradigms involving different competitive factors, such as physical strength vs psychological features, muscular confrontation vs threat perception, and boldness vs timidity, may produce task-specific win-or-lose outcomes and lead to inconsistent ranking results. Here, we developed a food competition apparatus for mouse, in which contenders were a pair of mice eager to take over the same food pellet placed under a movable block in the middle of a narrow chamber where they were separated to the either right or left side. This Food Pellet Competition Test (FPCT) was designed to (1) provide researchers with a choice of new food competition paradigm and (2) expose psychological factors influencing the establishment and/or expression social status in mice by avoiding direct physical competition between contenders. Meanwhile, we wanted to evaluate the consistency of social ranking results between FPCT and typically available space competition paradigms--tube test and warm spot test (WST). We hypothesized inconsistency of rankings of mice tested by FPCT, tube test and WST as they possess different targets for mice to compete and different factors determining competitiveness. Interestingly, application of FPCT in combination with tube test and WST discovered unexpected consistency of mouse social competitivity and rankings in a grouped male or female mice that were housed in an either 2-or 3-member cage, most likely indicating that the status sense of animals is part of a comprehensive identify of self-recognition of individuals in an established social colony. Furthermore, the FPCT may facilitate researches on social organization and competition, given its reliability, validity, and ease of use.